Review



5x fish skin gelatin blocking agent  (Biotium)


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    Structured Review

    Biotium 5x fish skin gelatin blocking agent
    Reagents and tools table
    5x Fish Skin Gelatin Blocking Agent, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5x+fish+skin+gelatin+blocking+agent/Fish+Gelatin+Blocking+Agent/pmc11791070-102-0-7
    Average 93 stars, based on 34 article reviews
    5x fish skin gelatin blocking agent - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Oncogenic PIK3CA corrupts growth factor signaling specificity"

    Article Title: Oncogenic PIK3CA corrupts growth factor signaling specificity

    Journal: Molecular Systems Biology

    doi: 10.1038/s44320-024-00078-x

    Reagents and tools table
    Figure Legend Snippet: Reagents and tools table

    Techniques Used: Knock-Out, Recombinant, Western Blot, Mass Cytometry, Sequencing, Clone Assay, Mutagenesis, Cell Culture, Membrane, Transfection, Lysis, Extraction, Protease Inhibitor, Sterility, Gel Extraction, Reverse Transcription, SYBR Green Assay, Blocking Assay, Staining, Software, Cytometry, Real-time Polymerase Chain Reaction, Microscopy

    Related Articles

    Blocking Assay:

    Article Title: Oncogenic PIK3CA corrupts growth factor signaling specificity
    Article Snippet: .. Next, 75 μl of DPBS was added, followed by 19 μl of 5X fish skin gelatin blocking agent (Biotium #22010) diluted in PBS/T (PBS with 0.05 % Tween-20) with 0.5% Triton-X100 (5X concentration, diluted to 0.1 % once added to the DPBS). .. The cells were left to permeabilize and block for 10 min, after which the block/perm solution was removed and replaced with DPBS supplemented with Phalloidin iFlouor 555 (Abcam #ab176756) and HCS CellMask Blue (Thermo Fisher Scientific #H32720), both diluted 1:1000.

    Article Title: Oncogenic PIK3CA corrupts growth factor signaling specificity
    Article Snippet: PowerUPTM SYBRTM Green Master Mix , Thermo Fisher Scientific , #A25742. .. 5X fish skin gelatin blocking agent , Biotium , #22010. .. Tween® 20, Molecular Biology Grade , Promega , #H5152.

    Concentration Assay:

    Article Title: Oncogenic PIK3CA corrupts growth factor signaling specificity
    Article Snippet: .. Next, 75 μl of DPBS was added, followed by 19 μl of 5X fish skin gelatin blocking agent (Biotium #22010) diluted in PBS/T (PBS with 0.05 % Tween-20) with 0.5% Triton-X100 (5X concentration, diluted to 0.1 % once added to the DPBS). .. The cells were left to permeabilize and block for 10 min, after which the block/perm solution was removed and replaced with DPBS supplemented with Phalloidin iFlouor 555 (Abcam #ab176756) and HCS CellMask Blue (Thermo Fisher Scientific #H32720), both diluted 1:1000.



    Similar Products

    93
    Biotium 5x fish skin gelatin blocking agent
    Reagents and tools table
    5x Fish Skin Gelatin Blocking Agent, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5x+fish+skin+gelatin+blocking+agent/Fish+Gelatin+Blocking+Agent/pmc11791070-102-0-7
    Average 93 stars, based on 1 article reviews
    5x fish skin gelatin blocking agent - by Bioz Stars, 2026-09
    93/100 stars
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    Reagents and tools table

    Journal: Molecular Systems Biology

    Article Title: Oncogenic PIK3CA corrupts growth factor signaling specificity

    doi: 10.1038/s44320-024-00078-x

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: 5X fish skin gelatin blocking agent , Biotium , #22010.

    Techniques: Knock-Out, Recombinant, Western Blot, Mass Cytometry, Sequencing, Clone Assay, Mutagenesis, Cell Culture, Membrane, Transfection, Lysis, Extraction, Protease Inhibitor, Sterility, Gel Extraction, Reverse Transcription, SYBR Green Assay, Blocking Assay, Staining, Software, Cytometry, Real-time Polymerase Chain Reaction, Microscopy